Thermo Fisher Scientific Phospho-PDHA1 (Ser293) Recombinant Rabbit Monoclonal Antibody (6E3J0)

Thermo Fisher Scientific Phospho-PDHA1 (Ser293) Recombinant Rabbit Monoclonal Antibody (6E3J0)

Applications and Tested Dilution

• Western Blot (WB): 1:2,000–1:20,000
• ELISA: 1 µg/mL
• Immunoprecipitation (IP): 0.5 µg–4 µg antibody for 200 µg–400 µg extracts

Product Specifications

Product Specific Information

Immunogen sequence: GHSMSD

Target Information

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the conversion of pyruvate to acetyl-CoA and CO₂, providing the primary link between glycolysis and the tricarboxylic acid (TCA) cycle.
The PDH complex consists of multiple copies of three enzymatic components:

• Pyruvate dehydrogenase (E1)
• Dihydrolipoamide acetyltransferase (E2)
• Lipoamide dehydrogenase (E3)

The E1 enzyme is a heterotetramer of two alpha and two beta subunits. The PDHA1 gene encodes the E1 alpha 1 subunit containing the E1 active site and is essential for PDH complex function. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.