Thermo Fisher Scientific HIF-1 alpha Monoclonal Antibody (Mgc3), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1.0 µg/test

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Mgc3

Immunogen

Human HIF-1 alpha amino acids 530-826. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HIF1A,uniProtId:Q16665-1,ncbiNodeId:9606,antigenRange:530-826,antigenLength:826,antigenImageFileName:12-7528-82_HIF1A_Q16665-1_House_mouse.svg,antigenImageFileNamePDP:12-7528-82_HIF1A_Q16665-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE PE PE

View additional formats

• APC
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802189

Product Specific Information

Description: This Mgc3 monoclonal antibody detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT or the related HIF-2 alpha.

Mgc3 has been successfully used in western blotting, immunofluorescence, immunocytochemistry, immunoprecipitation, flow cytometry and gel shift procedures. By western blot, this antibody detects a ~116 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. Immunofluorescence staining of HIF-1 alpha in COS-7 cells with clone Mgc3 yields nuclear staining after exposing cells to 1% oxygen for 4 hours. In gel shift assay experiments, the Mgc3 antibody detected only mouse and not human HIF-1 alpha.

Applications Reported: This Mgc3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Mgc3 antibody has been tested by flow cytometric analysis of DFOA stimulated HeLa cells using the methanol fixation protocol. This may be used at less than or equal to 1.0 µg/mL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

Target Information

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.