Thermo Fisher Scientific COPB2 Polyclonal Antibody
Thermo Fisher Scientific의 COPB2 Polyclonal Antibody는 인간 및 생쥐에 반응하며, WB, IHC, IP 등에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 항체로 1 mg/mL 농도. 골지체 관련 단백질 복합체 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 856 to 906 of human Coatomer Protein Complex, Subunit Beta 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, predicted to react with Rat, Bovine, and Orangutan
Target Information
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non–clathrin-coated vesicles. These vesicles mediate biosynthetic protein transport from the ER through the Golgi to the trans-Golgi network. The coatomer complex is required for budding from Golgi membranes and is essential for retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins. The complex also influences Golgi structural integrity, as well as processing, activity, and endocytic recycling of LDL receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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