Thermo Fisher Scientific IDH3B Polyclonal Antibody
인간 IDH3B 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 보존됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,500–1:5,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human IDH3B. Recombinant protein control fragment (Product #RP-99328). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2642623 |
Product Specific Information
Immunogen sequence:
VKEVFKAAAV PVEFQEHHLS EVQNMASEEK LEQVLSSMKE NKVAIIGKIH TPMEYKGELA SYDMRLRRKL DLFANVVHVK SLPGYMTRHN N
Sequence identity:
Highest antigen sequence identity to the following orthologs:
- Mouse: 96%
- Rat: 96%
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one utilizing NAD(+) as the electron acceptor and the other NADP(+).
Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent enzymes localized in the mitochondrial matrix, and two NADP(+)-dependent enzymes (one mitochondrial, one cytosolic).
NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer composed of two alpha subunits, one beta subunit, and one gamma subunit.
The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Three alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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