Thermo Fisher Scientific SSEA3 Monoclonal Antibody (MC-631), DyLight 650

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10-1:100

-

Flow Cytometry (Flow)

1:50-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgM

Class

Monoclonal

Type

Antibody

Clone

MC-631

Immunogen

Fischer rat immunized with 4-8 cell stage mouse embryos

Conjugate

DyLight™ 650 DyLight™ 650 DyLight™ 650

View additional formats

• Unconjugated
• DyLight 488
• PE
• Request custom conjugation

Excitation/Emission Max

651/673 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography - MBP

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536684

Product Specific Information

MA1-020-D650 has been successfully used in ICC/IF and flow cytometry applications on human samples.

Target Information

SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.