Thermo Fisher Scientific TEM5 Polyclonal Antibody
TEM5 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC에 적합합니다. Human과 Rat 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 냉장 보관(2–8°C) 권장, 장기 보관 시 -20°C 보관 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunohistochemistry (IHC) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 15 amino acid peptide near the carboxy terminus of the human TEM5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152123 |
Product Specific Information
- A suggested positive control is human bladder tissue lysate.
- PA5-20441 can be used with blocking peptide PEP-0558.
Target Information
Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogenesis, which are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for the development of anti-angiogenic therapies. These include TEM1, TEM5, TEM7, and TEM8.
TEM5 is a member of the adhesion family of G protein-coupled receptors and is localized on the surface of endothelial cells. Unlike TEM1, TEM7, and TEM8, which span the membrane once, TEM5 is a seven-pass transmembrane receptor. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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