Thermo Fisher Scientific Phospho-IRS1 (Tyr896) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000
• Publications: View 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:10–1:100
• Publications: View references

Product Specifications

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1), and interleukin 4 (IL-4). IRS-1 undergoes phosphorylation on serine, threonine, and tyrosine residues in various tissues. Casein kinase II mediates part of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site. Casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374, Ser 641), with the motif around Ser 270 located in the phosphotyrosine binding domain responsible for interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.