Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합 F(ab`)2 염소 항-인간 IgG(H+L) 2차 항체로 Western blot, IHC, ELISA에 적합합니다. 사람 IgG에 특이적이며 소, 마우스, 토끼 단백질과 교차 반응이 없습니다. 동결건조 형태로 제공되며 높은 특이성과 신뢰성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light Chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535939 |
| Target | IgG |
| Cross Adsorption | Against bovine, mouse and rabbit serum proteins |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
- Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Product is overfilled to ensure complete recovery.
- Centrifuge to remove particulates.
- Prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum proteins or serum proteins from bovine, mouse, or rabbit.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of human IgG targets.
Secondary antibodies provide versatility and enhanced sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, they are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified via immunoaffinity chromatography or modified by antibody fragmentation and label conjugation.
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 633
- Alexa Fluor 647
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- AP
- Biotin
- FITC
- Rhodamine
- TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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