Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG (H+L) Secondary Antibody, AP
Goat에서 유래한 F(ab`)2 fragment 형태의 anti-Human IgG (H+L) 2차 항체로, AP conjugate를 포함합니다. Western blot, IHC, ELISA 등 다양한 응용에 사용 가능하며, 높은 특이성과 감도를 제공합니다. 연구용으로만 사용됩니다.
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- A24467
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Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG (H+L) Secondary Antibody, AP
Applications 및 권장 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
제품 사양
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term); -20°C (long term, avoid freeze/thaw) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535936 |
| Target | IgG |
| Antibody Form | F(ab`)2 Fragment |
추가 포맷 옵션
- Alexa Fluor 488
- Alexa Fluor 647
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- Biotin
- FITC
- HRP
- PE
- Qdot 655
- Rhodamine
- Texas Red
- TRITC
- Request custom conjugation
Product Specific Information
Each lot’s sensitivity is confirmed using ELISA, and specificity is verified by immunoelectrophoresis (IEP).
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of target molecules. Secondary antibodies provide versatility across detection systems (HRP, AP, fluorescence) and enable signal amplification since multiple secondary antibodies can bind to a single primary antibody. Typically, they are generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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