Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), PE-Cyanine5, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), PE-Cyanine5, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 21 publications

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Product Specifications

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Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 480
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue / Red / Yellow series
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

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Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG (50–80 kDa).
CD16 includes two genes:

• CD16A (FcγRIIIA): Expressed on macrophages and NK cells, peptide-anchored form
• CD16B (FcγRIIIB): Expressed on neutrophils, GPI-anchored monomeric form

Two polymorphisms of CD16B (NA-1, NA-2) exist; NA-2 homozygotes show lower phagocytic capacity.
CD16 binds IgG immune complexes, preferentially IgG1 and IgG3, initiating ADCC, phagocytosis, degranulation, and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for human peripheral blood cells at 5 µL (0.25 µg)/test
  • Each test stains cells in 100 µL final volume (10⁵–10⁸ cells/test)

Light Sensitivity: Protect from light (photo-induced oxidation).
Fixation:

• Store in IC Fixation Buffer (Product #00-822-49)
• Or 1-step Fix/Lyse Solution (Product #00-5333-54)
• Up to 3 days at 4°C in the dark with minimal brightness loss.

Excitation: 488–561 nm
Emission: 667 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

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Target Information

CD16 encodes an Fc receptor for IgG, involved in immune complex clearance and antibody-dependent cellular cytotoxicity.

• Gene: FCGR3A (chromosome 1)
• Expression:
  • NK cells (transmembrane glycoprotein form)
  • Neutrophils (PI-anchored form via FCGR3B)
• Clinical Relevance: Associated with Immunodeficiency 20, Herpes Zoster, and susceptibility to lupus and recurrent viral infections.
• Variants: Alternative splicing yields multiple isoforms.

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For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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