Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), PE-Cyanine5, eBioscience
마우스 CD11b를 인식하는 M1/70 단클론 항체로, PE-Cyanine5로 결합된 형광 표지 항체입니다. 유세포분석(Flow Cytometry)에 적합하며, 인간 CD11b에도 교차 반응합니다. 액상 형태로 제공되며 4°C 암소 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 290 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/70 |
| Conjugate | PE-Cyanine5 |
| Excitation/Emission Max | 568/666 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468713 |
Additional Available Formats
Unconjugated, Alexa Fluor 488, 532, 660, 700, APC, APC-eFluor 780, Biotin, Brilliant UV/BV series, eFluor series, FITC, Functional Grade, NovaFluor series, PE, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright series.
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Product Specific Information
Description:
The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b associates with CD18 to form the αMβ2 integrin (Mac-1), binding to CD54 (ICAM-1), C3bi, and fibrinogen. It is expressed on macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells in the peritoneal cavity. The antibody also cross-reacts with human CD11b, detecting it on monocytes, granulocytes, and some NK cells.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. Use ≤0.125 µg per test (defined as the antibody amount staining a 100 µL cell sample). Optimal titration is recommended.
Light Sensitivity:
Tandem dye is sensitive to photo-induced oxidation. Protect from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation/Emission:
488–561 nm / 667 nm
Laser: Blue, Green, Yellow-Green
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD11b (integrin alpha-M, ITGAM):
A 165 kDa adhesion molecule forming a heterodimer with CD18 (integrin beta-2), known as Mac-1 or CR3. Expressed on monocytes/macrophages, granulocytes, NK cells, dendritic cells, and microglia.
Functions as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, mediating cell-cell and cell-matrix interactions.
Expression varies across cell types — 8% of spleen cells, 44% of bone marrow cells, <1% of thymocytes — and is a common microglial marker.
Associated with systemic lupus erythematosus and ITGAM-related autoimmune susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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