Thermo Fisher Scientific High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit
혈청, 혈장, 세포 등에서 HDL-C를 정량 측정하는 고감도 색도 분석 키트. 간단하고 빠른 프로토콜로 20분 내 결과 확인 가능. 546 nm에서 흡광도를 측정하여 HDL-C 농도 계산. 안정적이고 재현성 높은 품질 관리 기준 적용.
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Invitrogen™ High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit
The High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit is a reliable and sensitive assay designed for quantitative measurement of HDL-C in serum, plasma, culture supernatant, tissue, and cells.
HDL-C is an anti-atherosclerotic lipoprotein that transports cholesterol from extrahepatic tissues to the liver for metabolism and excretion of bile. Its plasma level is negatively correlated with cardiovascular disease risk.
Assay Principle
The assay is based on the enzymatic reaction of HDL-C with cholesterol esterase and cholesterol oxidase, producing hydrogen peroxide.
Hydrogen peroxide reacts with 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound, which has a maximum absorbance at 546 nm.
HDL-C concentration is calculated by measuring OD at 546 nm.
Key Features
- Simple and fast protocol with minimal hands-on time
- Rapid results within short incubation period
- Broad range of kits covering key analytes and metabolic pathways
- Consistent performance with strict quality control criteria
Specifications
| 항목 | 내용 |
|---|---|
| 어세이 범위 | 0.065–3.8 mmol/L |
| 어세이 민감성 | 0.065 mmol/L |
| 검출 방법 | Colorimetric |
| 샘플 종류 | Cell Culture Supernatant, Plasma, Serum, Tissue Homogenates, Whole Cells |
| 사용 장비 | Microplate Reader |
| 인큐베이션 시간 | 10 min |
| 분석 간 CV | 5% |
| 분석 내 CV | 3% |
| 수량 | 96 tests |
| 샘플 부피 | 2.5 μL |
| 배송 조건 | Dry Ice, 2–8°C |
| 타겟 키트 이름 | High-density Lipoprotein Cholesterol (HDL-C) |
| 총 어세이 시간 | 20 min |
| Unit Size | Each |
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