Thermo Fisher Scientific IL-27/p28 Monoclonal Antibody (3H12.F10)
Mouse IL-27/p28 단백질을 인식하는 Rat monoclonal antibody로, WB, Flow Cytometry, ELISA에 사용 가능. 고순도 정제 및 안정한 액상 형태로 제공되며, -20°C에서 장기 보관 가능. IL-27 관련 면역반응 연구에 적합.
- 카탈로그번호
- 210-501-B54x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-27/p28 Monoclonal Antibody (3H12.F10)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Flow Cytometry (Flow) | 1 µg/mL |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3H12.F10 |
| Immunogen | Anti-IL-27/p28 monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
IL-27 / p28 antibody is purified by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse and rat p28 protein. Cross-reactivity with IL-27 from other sources has not been determined.
Target Information
IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30). IL-27 is produced by activated antigen-presenting cells (APCs) and mature dendritic cells.
IL-27 acts on NK cells and naive CD4+ T cells, and receptor expression analysis (WSX-1/TCCR and gp130) suggests it may also affect mast cells and monocytes. Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3, leading to their phosphorylation.
WSX-1/TCCR-deficient mice show impaired Th1 responses and increased susceptibility to L. monocytogenes infection, indicating IL-27’s essential role in initiating Th1 responses. IL-27 induces the Th1 transcription factor T-bet and suppresses the Th2 factor GATA-3, supporting Th1 differentiation.
Recent studies also show IL-27 has potent antitumor activity by enhancing CTL function, inhibiting tumor growth, and inducing regression of neuroblastoma tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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