Thermo Fisher Scientific CD11b Chimeric Recombinant Rat Monoclonal Antibody (5C6 (recombinant version))

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody specifically recognizes CD11b, also known as the integrin alpha M chain (MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells, and tissue macrophages.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex, also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3), is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, dendritic cells, and microglia.

CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. These interactions are critical for cell-cell and cell-matrix adhesion.

CD11b expression:

• 8% of spleen cells
• 44% of bone marrow cells
• <1% of thymocytes

It is commonly used as a microglial marker in nervous tissue. Expression increases during monocyte maturation and varies among tissue macrophages. Peritoneal macrophages express higher CD11b levels than splenic macrophages.

Diseases associated with CD11b dysfunction include systemic lupus erythematosus and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.