Thermo Fisher Scientific Human CDK1/cyclin A2, GST Tag Recombinant Protein
Human CDK1/cyclin A2 recombinant protein with GST tag for kinase assay applications. Co-expressed in baculovirus system, purified liquid form, validated in LanthaScreen™ assay. Stable at -80°C, optimized for research use only.
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- Protein
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- pk
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Thermo Fisher Scientific Human CDK1/cyclin A2, GST Tag Recombinant Protein
Applications
- Kinase Assay (KA)
Tested Dilution
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | Baculovirus |
| Amino Acid Sequence | 1–297 |
| Tag | GST-tag |
| Molecular Weight | 60.4–74.8 kDa |
| Class | Recombinant |
| Type | Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Purified |
| Storage Buffer | 50 mM Tris HCl (pH 7.5), 25% glycerol, 0.1 mM EGTA, 0.1 mM PMSF, 0.1 mM EDTA, 150 mM NaCl, 0.25 mM DTT |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
Product Specific Information
For maximum recovery, please spin prior to use.
Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use original packaging until exhausted.
For larger sizes, reduce freeze/thaw cycles by preparing aliquots (not below 20 µL).
Do not store kinase diluted.
Properly stored at -80°C, guaranteed for 6 months from date of purchase.
Recombinant full-length human CDK1 and Cyclin A2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Features:
- Validated in LanthaScreen™ Kinase Binding Assay
- Protocols available for simple and optimized use
- Multiple pack sizes available for convenience
Related Product:
LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant
Gene Accession Numbers: CDK1 (NP_001777.1), Cyclin A2 (NP_001228.1)
Target Information
CDK1 (Cell Division Control protein 1) is essential for the completion of START, initiating mitosis. It acts as the catalytic subunit of the M-Phase Promoting Factor, inducing mitotic entry in eukaryotic cells.
Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves CDK1 signaling and delays apoptosis onset.
CDK inhibitor NU2058 shows therapeutic potential in androgen-independent prostate cancer.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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