Thermo Fisher Scientific SARS Coronavirus Nucleocapsid Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:2,000

View 1 publication 1 publication

ELISA (ELISA)

1:100-1:2,000

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Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues 84-99 of putative SARS nucleocapsid.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1087203

Product Specific Information

The immunogen is 94%(15/16) homologous to SARS-COV-2 (COVID-19) Nucleocapsid protein. The cross reactivity is not empirically tested.

The antibody was tested on a cell line transfected with full-length SARS Nucleocapsid cDNA with a predicted molecular weight of 46 kDa.

Suggested positive control: transfected mouse melanoma cell lysate.

Target Information

Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). The nucleocapsid protein (N) was one of the structural proteins of SARS-CoV (N, S, sE, M). The severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid (N) protein is the most abundant protein in the virus-infected cells. The SARS-CoV-2 S gene has 76% amino acid similarity to the SARS-CoV S gene, and nonsynonymous mutations developed in the S protein as the SARS-CoV epidemic developed, reports have shown that the crystal structure of the SARS-CoV-2 nucleocapsid protein is similar to those of previously described coronavirus N proteins, but their surface electrostatic potential characteristics are distinct.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.