Thermo Fisher Scientific ETV5 Polyclonal Antibody
ETV5 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, ChIP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 4°C 단기 보관 및 -20°C 장기 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (IHC) | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| ChIP assay (ChIP) | 2.5 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 194–394 of Human ETV5 (UniProt ID: P41161-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.48 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7) with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547497 |
Product Specific Information
- Recommended positive control: ERM-transfected 293T cells
- Predicted reactivity: Mouse (98%), Rat (95%), Pig (98%), Chicken (88%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly before opening the vial.
Target Information
ETS variant 5 (ETV5) is a transcription factor of the ETS family, a divergent member of the winged helix-turn-helix superfamily. It is expressed in brain, placenta, and other tissues involved in development.
ERM is expressed in Sertoli cells and spermatogonial stem cells (SSCs). Knockout mouse studies indicate that ETV5 is required for SSC self-renewal but not differentiation, leading to progressive germ cell depletion in affected mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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