Thermo Fisher Scientific ETV5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

ChIP assay (ChIP)

2.5 µg/10^6 cells

-

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 194 and 394 of Human ERM if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ETV5,uniProtId:P41161-1,ncbiNodeId:9606,antigenRange:194-394,antigenLength:510,antigenImageFileName:PA5-30023_ETV5_P41161-1_Rabbit.svg,antigenImageFileNamePDP:PA5-30023_ETV5_P41161-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.48 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2547497

Product Specific Information

Recommended positive controls: ERM-transfected 293T.

Predicted reactivity: Mouse (98%), Rat (95%), Pig (98%), Chicken (88%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ETS variant 5, transcription factor of the ETS family, divergent member of the winged helix-turn-helix superfamily, expressed in brain, placenta and other tissues involved in development. Tissue specificity: Ubiquitous. ERM is expressed in Sertoli cells and spermatogonial stem cells (SSCs). Studies on knockout mice have shown that it is required for SSC self-renewal but not differentiation, thus affected mice exhibit progressive germ cell depletion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.