Thermo Scientific™

Kinase Tracer 222, 50 μM

Kinase Tracer 222 is an Alexa Fluor™ 647 dye-labeled, ATP-competitive kinase inhibitor scaffold for use in the LanthaScreen™ Eu Kinase자세히 알아보기

Kinase Tracer 222 is an Alexa Fluor™ 647 dye-labeled, ATP-competitive kinase inhibitor scaffold for use in the LanthaScreen™ Eu Kinase Binding Assay.

The LanthaScreen™ Eu Kinase Binding Assay
• Is easily optimized
• Measures inhibitor binding
• Performs simple off-rate measurements

Easily Screen for Kinase Binding
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor™ conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (TR-FRET), while displacement of the tracer with a kinase inhibitor results in a loss of TR-FRET.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

사양

형식384-well plate

용도(애플리케이션)TR-FRET

수량25 μL

제품 유형Kinase Tracer 222

검출 방법Fluorescence

용도(장비)Microplate Reader

판독Kinetic

배송 조건Dry Ice

타겟Kinases (Multiple)

라벨 또는 염료Alexa Fluor™ 647

색상Red

제품라인LanthaScreen™

방출665

여기340

Unit SizeEach