Thermo Fisher Scientific Coagulation Factor VII/F7 Monoclonal Antibody (F7, 3511)
인간 Coagulation Factor VII(F7)을 인식하는 Mouse Monoclonal Antibody로, IHC 및 Peptide Array에 사용 가능. Recombinant F7 fragment(aa 366–466)를 면역원으로 제작. Protein A/G 정제, 200 µg/mL 농도, PBS buffer에 보존. 연구용으로만 사용.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | F7, 3511 |
| Immunogen | Recombinant fragment (around aa 366–466) of human F7 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Coagulation factor VII is a vitamin K-dependent protein essential for hemostasis. It circulates in the blood as a zymogen and is converted to an active form by factor IXa, Xa, XIIa, or thrombin through minor proteolysis. Upon activation, a heavy chain with a catalytic domain and a light chain with two EGF-like domains are formed and linked by a disulfide bond. In the presence of factor III and calcium ions, activated factor VII further activates the coagulation cascade by converting factor IX to IXa and/or factor X to Xa.
Defects in F7 production can lead to coagulopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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