Thermo Fisher Scientific Coagulation Factor VII/F7 Monoclonal Antibody (F7, 3513)
인간 Coagulation Factor VII(F7)을 인식하는 단클론 항체로, IHC(P) 및 Peptide Array에 사용 가능. 단백질 A/G로 정제된 액상 형태이며, PBS(pH 7.4) 버퍼에 보존제 없이 제공. 혈액응고 연구 및 F7 관련 단백질 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | F7, 3513 |
| Immunogen | Recombinant fragment (around aa 366–466) of human F7 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is tested in direct ELISA.
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
Coagulation factor VII is a vitamin K-dependent factor essential for hemostasis. It circulates in the blood as a zymogen and is activated by factor IXa, Xa, XIIa, or thrombin through limited proteolysis. Upon activation, a heavy chain (catalytic domain) and a light chain (two EGF-like domains) are formed and linked by a disulfide bond. In the presence of factor III and calcium ions, activated factor VII further activates the coagulation cascade by converting factor IX to IXa and/or factor X to Xa. Defects in F7 production can cause coagulopathy.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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