Thermo Fisher Scientific NNT1 Polyclonal Antibody, PeproTech
Human NNT-1/BCSF-3 인식용 Rabbit Polyclonal Antibody로 Western blot 및 ELISA에 적합. 항원 친화 크로마토그래피로 정제된 고순도 제품. 신경영양 및 B세포 자극 관련 연구에 활용 가능. 연구용으로만 사용.
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Thermo Fisher Scientific NNT1 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human NNT-1/BCSF-3 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929714 |
Product Specific Information
AA Sequence of recombinant protein:
MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human NNT-1/BCSF-3. Anti-Human NNT-1/BCSF-3-specific antibody was purified by affinity chromatography employing an immobilized Human NNT-1/BCSF-3 matrix.
Sandwich ELISA:
To detect Human NNT-1/BCSF-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NNT-1/BCSF-3 (500-P186Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human NNT-1/BCSF-3.
Western Blot:
To detect Human NNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human NNT-1/BCSF-3 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
NNT-1/BCSF-3 is a neurotrophic factor with B-cell-stimulating capabilities. Expressed in the lymph nodes and spleen, NNT-1/BCSF-3 activates glycoprotein 130 (gp130) and leukemia inhibitory factor receptor member b (LIFR-b) by binding and inducing the tyrosine phosphorylation of these receptors. In vitro, it supports the survival of chick embryo motor and sympathetic neurons. In mice, NNT-1/BCSF-3 induces serum amyloid A, causes body weight loss, and B cell hyperplasia associated with increases in serum IgG and IgM.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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