Thermo Fisher Scientific NeutrAvidin Protein, DyLight 405
DyLight 405 형광으로 표지된 NeutrAvidin 단백질로 높은 비특이적 결합 억제와 강력한 비오틴 결합력을 제공합니다. 다양한 면역실험(WB, IHC, ICC, IP)에 적합하며 4°C에서 안정적으로 보관 가능합니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- 22831
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific NeutrAvidin Protein, DyLight 405
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:25,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Type | Protein |
| Conjugate | DyLight™ 405 |
| Excitation/Emission Max | 400/423 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Catalog No. 22831 contains 1 mL of protein in clear liquid at a concentration of 1 mg/mL.
Target Information
Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles, and amphibia. The biotin-binding protein contains four identical subunits with a combined mass of 67,000–68,000 daltons.
Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of approximately 60,000 daltons. This modification reduces carbohydrate-based lectin binding to undetectable levels while retaining biotin-binding affinity.
NeutrAvidin Protein provides a neutral pI to minimize nonspecific adsorption and retains lysine residues available for derivatization or conjugation. It exhibits the lowest nonspecific binding among known biotin-binding proteins, with a specific activity for biotin-binding of approximately 14 µg/mg of protein, near the theoretical maximum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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