Thermo Fisher Scientific HMCES Polyclonal Antibody
Rabbit polyclonal antibody against human HMCES for WB, IHC, and ICC applications. Recognizes abasic sites in ssDNA, supporting genome integrity studies. Antigen affinity purified, supplied as liquid form with PBS buffer and glycerol. Suitable for resea...
- 카탈로그번호
- PA560876
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific HMCES Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human HMCES. Recombinant protein control fragment (Product #RP-100261). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2642472 |
Product Specific Information
Immunogen sequence:
WRLLTMAGIF DCWEPPEGGD VLYSYTIITV DSCKGLSDIH HRMPAILDGE EAVSKWLDFG EVSTQEALKL IHPTENITFH AVSSVVNNS
Highest antigen sequence identity to the following orthologs:
- Mouse: 87%
- Rat: 88%
Target Information
HMCES acts as a sensor of abasic sites in single-stranded DNA (ssDNA), preserving genome integrity by promoting error-free repair of abasic sites. It binds abasic sites at replication forks and forms a covalent cross-link with DNA via a thiazolidine linkage between the lesion and its catalytic cysteine residue. The resulting DNA-protein cross-link is degraded by the proteasome.
This mechanism protects abasic sites from error-prone polymerases and endonucleases that could cause mutations or double-strand breaks. HMCES also participates in class switch recombination (CSR) in B-cells by binding ssDNA overhangs and promoting DNA double-strand break repair through the microhomology-mediated alternative-end-joining (Alt-EJ) pathway.
Additionally, HMCES mediates autocatalytic processing of its N-terminal methionine to expose the catalytic cysteine.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.