Thermo Fisher Scientific Human/Mouse/Rat GDF-11 Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

View 2 publications 2 publications

Dot blot (DB)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 10 publications 10 publications

Miscellaneous PubMed (Misc)

-

View 11 publications 11 publications

Product Specifications

Species

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

NLGLDCDEHS SESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGQCEYM FMQKYPHTHL VQQANPRGSA GPCCTPTKMS PINMLYFNDK QQIIYGKIPG MVVDRCGCS

Molecular weight

25 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Assay 1: Determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.08-0.10 ug/ml. Assay 2: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1cells. The ED50 for this effect is 8-10 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

120-11-1MG will be provided as 2 x 500 µg (120-11-500UG).

Recombinant Human/Murine/Rat GDF-11 is a 25.0 kDa disulfide-linked homodimer containing two 109 amino acid polypeptide chains. It is highly homologous to myostatin/GDF-8, sharing 90% amino acid sequence identity.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

GDF11 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.