Thermo Fisher Scientific SKA3 Polyclonal Antibody
인간 SKA3 단백질을 인식하는 토끼 폴리클로날 항체. Western blot 및 immunoprecipitation에 적합. 항원 친화 크로마토그래피로 정제된 액상 형태. 세포 분열 시 방추체-키네토코어 복합체 연구용. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Assay-dependent
Immunoprecipitation (IP)
- 2–10 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 175 to 225 of Human Spindle and Kinetochore Associated Complex Subunit 3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Upon entry into mitosis, the cell’s microtubule (MT) network forms the mitotic spindle, enabling segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin, termed kinetochores (KT), are essential for proper attachment of chromosomes to spindle MTs.
A spindle and kinetochore complex composed of SKA1, SKA2, and SKA3 proteins is required for stable KT–MT interactions and timely anaphase onset. Depletion of SKA3 by siRNA delays anaphase transition, causing a prolonged metaphase-like state. These SKA3-depleted cells accumulate high levels of checkpoint protein Bub1 at kinetochores, indicating that the SKA complex is crucial for spindle checkpoint silencing and maintenance of chromosome cohesion during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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