Thermo Fisher Scientific SKA3 Polyclonal Antibody
Human SKA3 단백질을 표적으로 하는 Rabbit Polyclonal Antibody로, Western Blot 및 Immunoprecipitation에 적합. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 1 mg/mL 농도. 세포 분열 시 염색체 분리 및 키네토코어 연구에 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 2–10 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 362–412 of Human Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Upon entry into mitosis, the cell’s microtubule (MT) network forms the mitotic spindle, enabling segregation of paired chromosomes. Protein structures on centromeric chromatin, termed kinetochores (KT), are essential for proper chromosome attachment to spindle MTs.
The spindle and kinetochore complex (SKA complex)—comprising SKA1, SKA2, and SKA3—is required for stable KT-MT interactions and timely anaphase onset.
Depletion of SKA3 by siRNA delays the anaphase transition, resulting in a prolonged metaphase-like state. SKA3-deficient cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, indicating that the SKA complex plays a key role in spindle checkpoint silencing and chromosome cohesion during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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