Thermo Fisher Scientific PSMD2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:250

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C A(278) T N R P D T L D P A L L R P G R(294) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PSMD2,uniProtId:Q13200-1,ncbiNodeId:9606,antigenRange:278-294,antigenLength:908,antigenImageFileName:PA1-962_PSMD2_Q13200-1_Rabbit.svg,antigenImageFileNamePDP:PA1-962_PSMD2_Q13200-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2172782

Product Specific Information

PA1-962 detects proteasome 19S subunit 10B from canine, hamster, human, mouse and rat tissues and cells.

PA1-962 has been successfully used in Western blot procedures. By Western blot, this antibody detects ~42, 46, 48, and 52 kDa proteins which correspond to subunit 10B and probably the combined signal from 4, 6B, 7, and 8 subunit proteins.

PA1-962 immunizing peptide corresponds to amino acid residues 278-294 of human proteasome 19S subunit 10B. This sequence is conserved in 19S protein subunits S4 (53 kDa), S6A (53 kDa), S6B (46 kDa), S7 (47 kDa), and S8 (~45 kDa). PA1-962 immunizing peptide (Cat. # PEP-107) is available for use in neutralization and control experiments.

Target Information

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.