Thermo Fisher Scientific Cytokeratin 1 Polyclonal Antibody
Human reactive polyclonal antibody targeting Cytokeratin 1, suitable for WB and IHC(P) applications. Rabbit IgG, affinity purified, unconjugated form. Stable liquid formulation with PBS and sucrose; store at -20°C to avoid freeze-thaw cycles.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1.25–2.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide directed towards the middle region of human KRT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 2% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2884261 |
Product Specific Information
Immunogen sequence:
GSYGSGSSSG GYRGGSGGGG GGSSGGRGSG GGSSGGSIGG RGSSSGGVKS
For short-term use, store at 2–8°C up to 1 week. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Predicted homology:
Dog: 86%
Human: 100%
Rabbit: 93%
Rat: 93%
Target Information
Cytokeratins are a subfamily of intermediate filament proteins characterized by remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between approximately 40 kDa and 68 kDa and have isoelectric points between pH 4.9 and 7.8.
Individual human cytokeratins are numbered 1 to 20. Various epithelia in the human body express cytokeratins that are characteristic of the epithelium type and related to its degree of maturation or differentiation. Cytokeratin subtype expression patterns are increasingly used to distinguish different types of epithelial malignancies.
Cytokeratin antibodies assist in the differential diagnosis of tumors using immunohistochemistry on tissue sections and are also useful tools in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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