Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), PerCP-Cyanine5.5, eBioscience
Thermo Fisher의 CD127 Monoclonal Antibody (A7R34), PerCP-Cyanine5.5 형광 표지 항체로 마우스 CD127(IL-7 receptor α chain)을 검출. Flow cytometry에 최적화되어 있으며, 높은 특이성과 안정성 제공. 연구용으로 면역세포 발달 및 활성 연구에 활용 가능.
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Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), PerCP-Cyanine5.5, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 35 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PerCP-Cyanine5.5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A7R34 |
| Conjugate | PerCP-Cyanine5.5 |
| Excitation / Emission Max | 489 / 679 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_906212 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 561
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- FITC
- Functional Grade
- NovaFluor Red 700
- NovaFluor Red 710
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 645
- Request custom conjugation available
Product Specific Information
The A7R34 monoclonal antibody reacts with mouse CD127, the high-affinity alpha subunit of the mouse IL-7 receptor.
IL-7 receptor alpha chain is expressed by immature B cells in bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+) thymocytes, but not double-positive (CD4+CD8+) thymocytes. In peripheral tissues, mature T cells express CD127 at low levels.
A7R34 inhibits IL-7 binding to its receptor and is used in in vivo and in vitro studies to elucidate IL-7’s role in T and B cell development and activation.
Binding of A7R34 blocks SB/199 antibody binding, which also recognizes mouse CD127.
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD127 (Interleukin-7 receptor, IL-7R) is a glycoprotein regulating lymphopoiesis.
The active receptor is an alpha/gamma chain heterodimer.
- The gamma(c) chain also associates with the IL-2 receptor and activates signal transduction.
- The alpha chain determines specific signaling events via cytoplasmic molecules.
CD127 promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells.
Dysfunction of CD127 is associated with severe combined immunodeficiency (SCID) and T-cell negative/B-cell negative/NK-positive SCID.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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