
Thermo Fisher Scientific Mouse CD96 His-tag Recombinant Protein
Thermo Fisher Scientific의 Mouse CD96 His-tag 재조합 단백질은 HEK293 세포에서 발현된 고순도 단백질로, ELISA, WB, IHC 등 다양한 면역분석에 적합합니다. 기능적 결합능을 검증하였으며, >90% 순도와 낮은 엔도톡신 수준을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Mouse |
| Expression System | HEK293 cells |
| Amino Acid Sequence | Mouse CD96, amino acids Met1–Met536 (Accession # Q3U0X8) with a C-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 58 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >90% by SDS-PAGE |
| Endotoxin Concentration | <1.0 EU/µg |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Activity
- Measured by its binding ability in a functional ELISA.
- Immobilized Mouse CD96 His-tag Recombinant Protein at 10 µg/mL (100 µL/well) can bind mouse PVR with a linear range of 6.4–160 ng/mL.
Product Specific Information
Reconstitution:
Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower.
It is recommended that the protein be aliquoted and used as soon as possible.
Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
CD96 (also known as TACTILE) is a cell adhesion receptor and a member of the Ig superfamily.
It is a single-pass transmembrane protein with three extracellular Ig-like domains and a proline/serine/threonine-rich region.
CD96 is expressed on resident monocytes and T cells at all developmental stages, including gamma-delta T cells, thymocytes, and NK cells.
In mice, this receptor is not expressed on B cells and does not exist as alternative splice variants.
CD96 binds CD155/poliovirus receptor and Nectin-1 to mediate NK cell adhesion and cytotoxicity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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