Thermo Fisher Scientific Mouse CD96 His-tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species

Mouse

Expression System

HEK293 cells

Amino acid sequence

Mouse CD96, amino acids Met1-Met536 (Accession # Q3U0X8) with a C-terminal His-tag

Tag

His-tag

Molecular weight

58 kDa

Class

Recombinant

Type

Protein

Purity

>90% by SDS-PAGE

Endotoxin concentration

<1.0 EU/µg

Activity

1. Measured by its binding ability in a functional ELISA.
2. Immobilized Mouse CD96 His-tag Recombinant Protein at 10 µg/mL (100 µL/well) can bind mouse PVR with a linear range of 6.4-160 ng/mL.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Product Specific Information

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Target Information

CD96 is a cell adhesion receptor also known as TACTILE. A member of the Ig superfamily, CD96 is a single pass transmembrane protein consisting of three extracellular Ig-like domains and a proline/serine/threonine rich region. CD96 is expressed on resident monocytes and T cells at all developmental stages (including gamma-delta T cells, thymocytes, and NK cells). In the mouse, this receptor is not expressed on B cells. Unlike in humans, mouse CD96 does not exist as alternative splice variants. Reports indicate that CD96 binds CD155/poliovirus receptor and Nectin-1 to mediate NK cell adhesion to target cells and cytotoxicity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.