Thermo Fisher Scientific c-Raf Polyclonal Antibody
c-Raf 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot 및 IHC(P)에서 사용 가능. Human, Mouse, Rat에 반응하며 고순도 항원 친화 크로마토그래피로 정제됨. 1 mg/mL 농도의 액상 형태로 제공되며, 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 211–260 of Human Raf-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2802993 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) that functions downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates MEK1 and MEK2, which in turn activate ERK1 and ERK2. Activated ERKs regulate gene expression involved in cell division, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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