Thermo Fisher Scientific Rat IL-2 Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Rat

Expression System

P. pastoris

Amino acid sequence

Rat IL-2, amino acids 21-155 (Accession # P04351)

Molecular weight

15.5 kDa

Class

Recombinant

Type

Protein

Purity

>95% by SDS-PAGE

Activity

ED50 = 3 ng/mL; determined by the dose-dependent proliferation of mouse CTLL-2 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

Mature rat IL-2 was expressed in Pichia pastoris, X-33. GenBank Accession # P04351, amino acids 21 - 155. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: APTSSPAKET. Purity is >95% by SDS-PAGE analyses.

Activity: The biological activity of recombinant rat IL-2 was determined by measuring the proliferation of mouse CTLL-2 cells. The ED50 (or 1 unit of activity) for this effect is typically 3 ng/mL.

Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.

Reconstitution and Dilution: Store lyophilized material at 4ºC. Reconstitute in 0.5 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70ºC, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.