Thermo Fisher Scientific TEM5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

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Immunohistochemistry (IHC)

20 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunocytochemistry (ICC/IF)

-

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Product Specifications

Species Reactivity

Human, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 14 amino acid synthetic peptide near the center of human TEM5. The immunogen is located within amino acids 560-610 of TEM5.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152277

Product Specific Information

A suggested positive control is rat kidney tissue lysate.

PA5-20442 can be used with blocking peptide PEP-0559.

Predicted species reactivity based on immunogen sequence: Mouse: (85%).

Target Information

Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.