Thermo Fisher Scientific TEM5 Polyclonal Antibody
TEM5 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC 등 다양한 응용에 적합합니다. 인간 및 랫드 반응성을 가지며, 항원 친화 크로마토그래피로 정제되었습니다. 4°C에서 보관하며, 종양 혈관 신생 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2–4 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | 20 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC-P) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 14 amino acid synthetic peptide near the center of human TEM5 (amino acids 560–610). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152277 |
Product Specific Information
- Suggested positive control: Rat kidney tissue lysate
- PA5-20442 can be used with blocking peptide PEP-0559
- Predicted species reactivity based on immunogen sequence: Mouse (85%)
Target Information
Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogenesis, which are crucial for solid tumor growth. TEMs localized on the cell surface and conserved across species are of particular interest for the development of anti-angiogenic therapies. These include TEM1, TEM5, TEM7, and TEM8.
TEM5 is a member of the adhesion family of G protein-coupled receptors and is localized on the surface of endothelial cells. Unlike TEM1, TEM7, and TEM8, which span the membrane once, TEM5 is a seven-pass transmembrane receptor. It is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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