Thermo Fisher Scientific SETD7 Polyclonal Antibody
SETD7 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, IHC, ChIP 등에 사용 가능. 인간 및 생쥐 반응성. 히스톤 H3 Lys-4 단일메틸화 효소 검출에 적합. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| ChIP assay (ChIP) | 1:10–1:500 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptides corresponding to residues G(131)EVNEDGEMTGEKIA(145) and G(336)YDHSPPGKSGPEAPEW(352) of human Set7/9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Whole serum, PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2185953 |
Product Specific Information
These sequences are 100% conserved between human, mouse, and rat.
Suggested positive control: HeLa whole cell extract, HeLa cell lysate.
Target Information
Histone methyltransferase that specifically monomethylates Lys-4 of histone H3.
H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation.
Plays a central role in the transcriptional activation of genes such as collagenase or insulin.
Recruited by IPF1/PDX-1 to the insulin promoter, leading to transcriptional activation.
Also methylates non-histone proteins such as p53/TP53, TAF10, and possibly TAF7.
Monomethylates Lys-189 of TAF10, increasing its affinity for RNA polymerase II.
Monomethylates Lys-372 of p53/TP53, stabilizing it and enhancing transcriptional activation.
[Source: UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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