Thermo Fisher Scientific EEA1 Monoclonal Antibody (1D4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

10 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

1D4

Immunogen

EEA1 (NP_003557, 1312 a.a. approximately 1411 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EEA1,uniProtId:Q15075-1,ncbiNodeId:9606,antigenRange:1312-1411,antigenLength:1411,antigenImageFileName:H00008411-M02A_EEA1_Q15075-1_House_mouse.svg,antigenImageFileNamePDP:H00008411-M02A_EEA1_Q15075-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Storage buffer

ascites

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: KVLELQRKLD NTTAAVQELG RENQSLQIKH TQALNRKWAE DNEVQNCMAC GKGFSVTVRR HHCRQCGNIF CAECSAKNAL TPSSKKPVRV CDACFNDLQG

Target Information

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.