Thermo Fisher Scientific AES Polyclonal Antibody, MaxPab

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AES (NP_945320.1, 1 a.a. approximately 264 a.a) full-length human protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MCHKNGFPQE GGITAAFLQK RKLRLSKNHR PARAKVTEHV RGTRPGRATA GPAASTRAAG SLFFDRWGNR GPAGCRGSSH LPQQLKFTTS DSCDRIKDEF QLLQAQYHSL KLECDKLASE KSEMQRHYVM YYEMSYGLNI EMHKQAEIVK RLNGICAQVL PYLSQEHQQQ VLGAIERAKQ VTAPELNSII RQQLQAHQLS QLQALALPLT PLPVGLQPPS LPAVSAGTGL LSLSALGSQA HLSKEDKNGH DGDTHQEDDG EKSD

Target Information

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.