Thermo Fisher Scientific UPF1 Monoclonal Antibody (4G3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

4G3

Immunogen

UPF1 (NP_002902.2, 1019 a.a. approximately 1116 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:UPF1,uniProtId:Q92900-1,ncbiNodeId:9606,antigenRange:1019-1116,antigenLength:1129,antigenImageFileName:H00005976-M01A_UPF1_Q92900-1_House_mouse.svg,antigenImageFileNamePDP:H00005976-M01A_UPF1_Q92900-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Storage buffer

ascites

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: GRQKNRFGLP GPSQTNLPNS QASQDVASQP FSQGALTQGY ISMSQPSQMS QPGLSQPELS QDSYLGDEFK SQIDVALSQD STYQGERAYQ HGGVTGLS

Target Information

Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons. Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm and was later shown to be a component of cytoplasmic P-bodies. Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery. Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.