Thermo Scientific™

Genomic DNA Purification Kit

Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various자세히 알아보기

Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 μg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

• Efficient—2 to 10 μg of genomic DNA from 0.2 mL of blood
• Fast—procedure takes approximately 20 minutes
• Universal—purifies genomic DNA from various sources
• Flexible—easy to scale up and down
• Safe—does not involve phenol extraction

Applications

• Purification of high-quality genomic DNA from:
• Whole blood or serum
• Cell culture
• Plant tissues
• Mammalian tissues
• Epithelium samples
• Bacteria Includes Lysis Solution Precipitation Solution NaCl Solution Detailed Protocol

사양

Isolation TechnologyOrganic Extraction

샘플 종류Bacteria, Cells, Plant, Serum, Tissue, Whole Blood, Blood

용리량20 to 100 μL

최종 제품 유형gDNA

용도(애플리케이션)PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization

고처리량 호환성Not High-throughput Compatible (Manual)

수량100 Preps

배송 조건Room Temperature

테스트 시간25 min.

시작 물질 양Bacteria: ≤20 mg
Cells: ≤106
Plant: ≤100 mg
Tissue: ≤30 mg
Serum: ≤200 μL
Whole Blood: ≤200 μL

Unit SizeEach