Thermo Fisher Scientific CYP27A1 Recombinant Rabbit Monoclonal Antibody (JG40-36)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Flow Cytometry (Flow)

1:50-1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JG40-36

Immunogen

Recombinant protein within Human CYP27A1 aa 400-531 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CYP27A1,uniProtId:Q02318-1,ncbiNodeId:9606,antigenRange:400-531,antigenLength:531,antigenImageFileName:MA5-34810_CYP27A1_Q02318-1_Rabbit.svg,antigenImageFileNamePDP:MA5-34810_CYP27A1_Q02318-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Wet ice

RRID

AB_2848718

Product Specific Information

Positive Control: Rat heart tissue, human liver tissue, human kidney tissue, human small intestine tissue, HepG2, LOVO.

Target Information

CYP27A1 participates in the degradation of cholesterol to bile acids in both the classic and acidic pathways. [1] It is the initiating enzyme in the acidic pathway to bile acids, yielding oxysterols by introducing a hydroxyl group to the carbon at the 27 position in cholesterol. In the acidic pathway, it produces 27-hydroxycholesterol from cholesterol whereas in the classic or neutral pathway, it produces 3beta-hydroxy-5-cholestenoic acid. It is also involved in the metabolism of vitamin D3. While CYP27A1 is present in many different tissues, its function in these tissues is largely uncharacterized. In macrophages, 27-hydroxycholesterol generated by this enzyme may be helpful against the production of inflammatory factors associated with cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.