Thermo Fisher Scientific PPBP Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESAD

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human NAP-2 (CXCL7). Anti-Human NAP-2 (CXCL7)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human NAP-2 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NAP-2 (CXCL7) (500-P03) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human NAP-2.

Western Blot:
To detect Human NAP-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human NAP-2 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P03BT-1MG will be provided as 2 × 500 µg.

Target Information

Members of the α-chemokine subfamily of inducible, secreted, pro-inflammatory cytokines contain a characteristic Cys-X-Cys motif and are chemotactic for neutrophils.
Platelet basic protein (PBP), a member of this family, resides in platelet α-granules and is released upon activation. Proteolytic cleavage of PBP generates several peptides, including mature PBP, CTAP III, β-thromboglobulin (β-TG), and NAP-2.
These peptides mediate inflammation and wound healing, with NAP-2 specifically activating chemotaxis and degranulation in neutrophils.
The human PBP gene maps to chromosome 4q12–q13.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.