Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 660, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 0.125 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 563 / 661 / 737 / 805
• Brilliant Violet 421 / 650 / 711 / 786
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue 585 / 610-70S / 660-120S / 760
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα).
CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes.
By itself, CD25 binds IL-2 with low affinity; however, when associated with CD122 (IL-2Rβ) and CD132 (common γ chain), it forms the high-affinity IL-2 receptor complex.
Binding of IL-2 to both high and low affinity receptors is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation, activation, and proliferation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes. Recommended ≤0.125 µg/test in 100 µL final volume. Cell number: 10⁵–10⁸ cells/test. Optimize titration for best results.
Fluorophore Info: Alexa Fluor 660 emits at 690 nm; suitable for spectral cytometers with red laser excitation (662 nm).

Target Information

CD25 (IL2RA) encodes the interleukin-2 receptor alpha chain, a cytokine receptor involved in T and B lymphocyte proliferation.
IL2RA forms a high-affinity receptor complex with IL2RB and IL2RG.
Homodimeric IL2RA forms a low-affinity receptor, while IL2RB homodimers yield a medium-affinity receptor.
IL2 expression in mature thymocytes is monoallelic, regulating precise gene expression.
IL2 acts as a growth and differentiation factor, stimulating B cell proliferation, enhancing NK cell activity, and inhibiting granulocyte macrophage colony formation.
Mutations in IL2RA are associated with interleukin-2 receptor alpha deficiency and immune dysregulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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