Thermo Fisher Scientific PBK Polyclonal Antibody
PBK 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal antibody로, WB, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 인간, 마우스, 랫트 반응성.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived human PBK recombinant protein (Position: N71–V322) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746901 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
PBK (TOPK) is a serine/threonine kinase related to dual specific mitogen-activated protein kinase (MAPKK). It is up-regulated in various hematological malignancies, participates in activating lymphoid cells, and supports testicular function (spermatogenesis). Mitotic phosphorylation is required for its catalytic activity, suggesting a role in lymphoid cell activation and spermatogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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