Thermo Fisher Scientific 14-3-3 beta Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody is predicted to react with rat and mouse based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is brain tissue.

Target Information

14-3-3 is a family of acidic and soluble proteins highly conserved across species, expressed in all eukaryotic cells.
Seven isoforms (beta, gamma, epsilon, eta, zeta, sigma, and tau/theta) are encoded by distinct genes in mammals and form homo- and hetero-dimeric cup-shaped structures.
14-3-3 interacts with more than 100 binding partners and regulates key proteins involved in signal transduction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology.
This protein is abundantly expressed in the brain and detected in cerebrospinal fluid of patients with various neurological disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.