Thermo Fisher Scientific Human Fibronectin Native Protein

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Class

Native

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1.5 mg/mL

Purification

SDS-PAGE

Storage buffer

0.02M HEPES, pH 7.4, with 0.15M NaCl

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

RP-43130 contains native human Fibronectin protein and may be used as a positive control in many immunological assays. Place in 37° C water bath to dissolve.

The product is of human blood/plasma origin. Although the starting material was tested prior to initiation of the manufacturing process, and was found negative or nonreactive for anti-HIV-1/2, HIV-1 antigen(s), HBsAg, STS, anti-HCV, anti-HBcore and anti-HTLV I & II, extreme caution should be used when handling this material as there is a margin of error in all tests.

This product is to be used for IN VITRO research purposes only, and is not intended for clinical or diagnostic use.

Reconstitute with 1.36 mL of water to achieve 1.5 mg/mL.

Target Information

Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.