Thermo Fisher Scientific p38 MAPK alpha Monoclonal Antibody (PS1009)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Product Specifications

Product Specific Information

82382 targets MAPK14 in Western blot, immunofluorescence, immunocytochemistry, and flow cytometry applications and shows reactivity with human, mouse, monkey, pig, S. cerevisiae, and rat samples.
82382 does not cross-react with either JNK/SAPK or p42/44 MAP Kinase.
MAPK14 has a predicted molecular weight of approximately 43 kDa.

Store at -20ºC and do not aliquot.

Target Information

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs.
The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals.
p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motif.

There are four isoforms of p38 MAPK, which differ in tissue expression and affinity for upstream activators and downstream effectors.
When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation.
Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK.
Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2, and other transcription factors through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.