Thermo Fisher Scientific His Tag Monoclonal Antibody (6G2A9), iFluor 555, THE

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1–4 µg/mL

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• FITC
• HRP
• iFluor 488
• iFluor 647
• Custom conjugation available

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make an antibody concentration of 0.5 mg/mL.

The His tag is a short amino acid sequence composed of histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. Anti-His tag antibodies are useful for the analysis of His-tagged proteins.

THE™ His Tag Antibody [iFluor 555], mAb, Mouse is the THE™ His Tag Antibody (A00186) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3–6. It is suitable for detecting His-tagged protein expression. iFluor 555 is a bright and photostable fluorescent dye, serving as an excellent alternative to Alexa Fluor 555.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at the N- or C-terminal regions of recombinant proteins to enable purification by immobilized metal affinity chromatography (IMAC).

Epitope tagging allows detection of recombinant proteins for which no specific antibody exists. His-tagged proteins bind strongly and selectively to Ni²⁺-NTA adsorbents, allowing efficient purification. His tag-specific antibodies facilitate detection or co-immunoprecipitation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.