Thermo Fisher Scientific His Tag Monoclonal Antibody (6G2A9), iFluor 488, THE

Applications and Tested Dilutions

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Product Specifications

Available Formats

• Unconjugated
• Biotin
• FITC
• HRP
• iFluor 555
• iFluor 647
• Request custom conjugation

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make the antibody concentration of 0.5 mg/mL.

The His tag is a short amino acid sequence consisting of histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins.

THE™ His Tag Antibody [iFluor 488], mAb, Mouse is THE™ His Tag Antibody (A00186) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4–8. It is suitable for detecting the expression level of His-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye, serving as an excellent alternative to Alexa Fluor 488.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). The His tag is commonly expressed at either the N- or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography (IMAC).

Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By selecting a specific epitope–protein combination, epitope tagging enables detection of proteins for which no antibody is available.

His-tagged proteins, containing histidine clusters at the carboxyl or amino terminus, are purified by metal chelate chromatography due to their strong and selective affinity for Ni²⁺. His tag-specific antibodies facilitate detection or co-immunoprecipitation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.