Thermo Fisher Scientific His Tag Monoclonal Antibody (6G2A9), iFluor 488, THE
His-tag 단백질 검출용 iFluor 488 결합 단일클론 항체로, 높은 형광 안정성과 밝기를 제공. ICC/IF 및 유세포분석에 적합하며, 마우스 IgG1 기반. 재조합 단백질의 발현 및 정제 확인에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–4 µg/mL |
| Flow Cytometry (Flow) | 1–4 µg/mL |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6G2A9 |
| Immunogen | Synthetic peptide HHHHHH coupled to KLH |
| Conjugate | iFluor™ 488 |
| Excitation / Emission Max | 491 / 516 nm |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 10 mg/mL BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
Product Specific Information
Reconstitute the lyophilized product with deionized water (or equivalent) to make the antibody concentration of 0.5 mg/mL.
The His tag is a short amino acid sequence consisting of histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins.
THE™ His Tag Antibody [iFluor 488], mAb, Mouse is THE™ His Tag Antibody (A00186) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4–8. It is suitable for detecting the expression level of His-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye, serving as an excellent alternative to Alexa Fluor 488.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). The His tag is commonly expressed at either the N- or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By selecting a specific epitope–protein combination, epitope tagging enables detection of proteins for which no antibody is available.
His-tagged proteins, containing histidine clusters at the carboxyl or amino terminus, are purified by metal chelate chromatography due to their strong and selective affinity for Ni²⁺. His tag-specific antibodies facilitate detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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