Thermo Fisher Scientific TOM20 Recombinant Rabbit Monoclonal Antibody (10G6I8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:160,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

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Immunocytochemistry (ICC/IF)

1:200-1:2,000

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ELISA (ELISA)

1 µg/mL

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Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

10G6I8

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TOMM20,uniProtId:Q15388-1,ncbiNodeId:9606,antigenRange:20-145,antigenLength:145,antigenImageFileName:MA5-34964_TOMM20_Q15388-1_Rabbit.svg,antigenImageFileNamePDP:MA5-34964_TOMM20_Q15388-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.10 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2848869

Product Specific Information

Immunogen sequence: YCIYFDRKRR SDPNFKNRLR ERRKKQKLAK ERAGLSKLPD LKDAEAVQKF FLEEIQLGEE LLAQGEYEKG VDHLTNAIAV CGQPQQLLQV LQQTLPPPVF QMLLTKLPTI SQRIVSAQSL AEDDVE

Target Information

The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.