Thermo Fisher Scientific GST Polyclonal Antibody, Rhodamine
GST 태그 단백질 검출용 로다민 결합 폴리클로날 항체. Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합. 높은 특이성과 안정성 제공. 연구용으로만 사용.
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Thermo Fisher Scientific GST Polyclonal Antibody, Rhodamine
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:60,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full-length GST isolated from Schistosoma japonicum |
| Conjugate | Rhodamine |
| Excitation / Emission Max | 573 / 591 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, as well as purified and partially purified Glutathione-S-Transferase (Schistosoma japonicum). Cross-reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Target Information
GST (Glutathione S-transferase) is a 26 kDa protein present in both eukaryotes and prokaryotes, catalyzing a variety of reactions. Glutathione S-transferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion proteins provide an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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