Thermo Fisher Scientific Tri-Methyl-Histone H3 (Lys9) Polyclonal Antibody
Tri-Methyl-Histone H3 (Lys9) 폴리클로날 항체로, 인간, 마우스, 랫트 시료에서 H3K9me3 검출에 적합. Western blot, ICC/IF, IP에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 0.5 mg/mL 농도의 액상 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide containing AR[Me3-K]ST in which Me3-K corresponds to trimethyl lysine 9 of human histone H3 (amino acid range 8–12 of the target protein). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: 3T3 Nuclear Extract
- Cellular location: Nucleus
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it forms the nucleosome structure that organizes DNA. Histones are key regulators of transcription, DNA repair, replication, and chromosomal stability. The N-terminal tail of histone H3 undergoes various post-translational modifications—such as methylation, acetylation, and phosphorylation—that influence chromatin structure and gene expression.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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